The converting a conventional polymer 3D printer equipment for bioprinting
Keywords:
3D printing, polymer, engineering and device.
Abstract
3D printing has become a versatile and powerful method in engineering, other disciplines are increasingly adapting it due to its tremendous potential beyond its typical applications. However, commercially available 3D printing systems are often expensive and elude wide deployment, including labs in low-resource settings. To address the limitations of conventional and commercially available technology, for which a polymer 3D printer was developed that can be installed in a single day, it is conveniently sized to fit into a standard, customizable, laminar flow hood. ultra-low cost and therefore accessible to a wide range of research laboratories or educational institutions. Accuracy and reproducibility of printing results using mainly polymers were evaluated to demonstrate their potential for diverse uses by printing various 2D and 3D objects, such that a parts list and 3D design files in STL format were presented. and STEP to rebuild the device.
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References
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Ab-Rahim, S., Selvaratnam, L., Raghavendran, H. R., and Kamarul, T. (2013). Chondrocyte-alginate constructs with or without TGF-β1 produces superior extracellular matrix expression than monolayer cultures. Mol. Cell. Biochem. 376, 11–20. doi: 10.1007/s11010-012-1543-0
Carter, S. D., Costa, P. F., Vaquette, C., Ivanovski, S., Hutmacher, D. W., and Malda, J. (2017). Additive biomanufacturing: an advanced approach for periodontal tissue regeneration. Ann. Biomed. Eng. 45, 12–22. doi: 10.1007/s10439-016-1687-2
Chhaya, M. P., Melchels, F. P., Holzapfel, B. M., Baldwin, J. G., and Hutmacher, D. W. (2015). Sustained regeneration of high-volume adipose tissue for breast reconstruction using computer aided design and biomanufacturing. Biomaterials 52, 551–560. doi: 10.1016/j.biomaterials.2015.01.025
Conrad, K. L., Shiakolas, P. S., and Yih, T. (2000). “Robotic calibration issues: accuracy, repeatability and calibration,” in Proceedings of the 8th Mediterranean Conference on Control and Automation (MED2000) (Rio: University of Patras).
Delalat, B., Harding, F., Gundsambuu, B. E. M., De-Juan-Pardo, Wunner, F. M., Wille, M. L., et al. (2017). 3D printed lattices as an activation and expansion platform for T cell therapy. Biomaterials 140, 58–68. doi: 10.1016/j.biomaterials.2017.05.009.
Duan, B., Hockaday, L. A., Kang, K. H., and Butcher, J. T. (2013). 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. J. Biomed. Mater. Res. A. 101, 1255–1264. doi: 10.1002/jbm.a.34420
Fahmy, M. D., Jazayeri, H. E., Razavi, M., Masri, R., and Tayebi, L. (2016). Three-dimensional bioprinting materials with potential application in preprosthetic surgery. J. Prosthodont. 25, 310–318. doi: 10.1111/jopr.12431
Faulkner-Jones, A., Fyfe, C., Cornelissen, D. J., Gardner, J., King, J., Courtney, A., et al. (2015). Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D. Biofabrication 7:044102. doi: 10.1088/1758-5090/7/4/044102
Goldstein, T. A., Epstein, C. J., Schwartz, J., Krush, A., Lagalante, D. J., Mercadante, K. P., et al. (2016). Feasibility of bioprinting with a modified desktop 3D printer. Tissue Eng. Part C Methods 22, 1071–1076. doi: 10.1089/ten.tec.2016.0286
Hong, N., Yang, G. H., Lee, J., and Kim, G. (2018). 3D bioprinting and its in vivo applications. J. Biomed. Mater. Res. B Appl. Biomater. 106, 444–459. doi: 10.1002/jbm.b.33826
Hunt, N. C., and Grover, L. M. (2013). Encapsulation and culture of mammalian cells including corneal cells in alginate hydrogels. Methods Mol. Biol. 1014, 201–210. doi: 10.1007/978-1-62703-432-6_14
Hunt, N. C., Shelton, R. M., and Grover, L. (2009). An alginate hydrogel matrix for the localised delivery of a fibroblast/keratinocyte co-culture. Biotechnol. J. 4, 730–737. doi: 10.1002/biot.200800292
Irvine, S. A., Agrawal, A., Lee, B. H., Chua, H. Y., Low, K. Y., Lau, B. C., et al. (2015). Printing cell-laden gelatin constructs by free-form fabrication and enzymatic protein crosslinking. Biomed. Microdevices. 17:16. doi: 10.1007/s10544-014-9915-8
Itoh, M., Nakayama, K., Noguchi, R., Kamohara, K., Furukawa, K., Uchihashi, K., et al. (2015). Scaffold-free tubular tissues created by a bio-3D printer undergo remodeling and endothelialization when implanted in Rat Aortae. PLoS ONE 10:e0136681. doi: 10.1371/journal.pone.0136681
Kim, S. H., Yeon, Y. K., Lee, J. M., Chao, J. R., Lee, Y. J., Seo, Y. B., et al. (2018). Precisely printable and biocompatible silk fibroin bioink for digital light processing 3D printing. Nat. Commun. 9:1620. doi: 10.1038/s41467-018-03759-y
Kuenzel, K., Mofrad, S. A., and Gilbert, D. F. (2017). “Phenotyping cellular viability by functional analysis of ion channels: GlyR-targeted screening in NT2-N cells,” in: Cell Viability Assays: Methods and Protocols. eds D.F. Gilbert and O. Friedrich (New York, NY; Springer New York), 205–214. doi: 10.1007/978-1-4939-6960-9_16
Landrain, T., Meyer, M., Perez, A. M., and Sussan, R. (2013). Do-it-yourself biology: challenges and promises for an open science and technology movement. Syst. Synth. Biol. 7, 115–126. doi: 10.1007/s11693-013-9116-4
Mannoor, M. S., Jiang, Z., James, T., Kong, Y. L., Malatesta, K. A., Soboyejo, W. O., et al. (2013). 3D printed bionic ears. Nano Lett. 13, 2634–2639. doi: 10.1021/nl4007744
Published
2022-11-18
Section
Artículos
